Abstract: Hydrogen peroxide (H2O2) is a vital oxidizing and signaling molecule extensively used across biomedical, environmental, and industrial fields. Accurate and rapid quantification of H₂O₂ is crucial for ensuring safety and process efficiency. In this work, a novel electrochemical sensor based on the synergistic integration of MXene materials and deep eutectic solvents (DESs) was developed for the sensitive and stable detection of H2O2. MXenes, with their exceptional electrical conductivity and catalytic activity, serve as an efficient electrocatalytic platform, while DESs enhance their dispersion, stability, and electrochemical performance by preventing oxidation and aggregation. The resulting MXene-DES composite electrode exhibited a wide linear detection range (0.01-8.0 mM) and a low detection limit (1.5 μM). Moreover, the sensor demonstrated excellent reproducibility, long-term stability, and successful application in detecting H2O2 in real serum samples, underscoring its potential for clinical diagnostics and environmental monitoring. This study provides a green and efficient strategy for developing next-generation electrochemical sensors by combining advanced two-dimensional materials with environmentally benign solvent systems.